12 games
| Date | Site | Opp AP | Opponent | UL AP | W/L | UL | Opp | OT | Overall | Conf | Attendance | City | State | Stadium | Event |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 09/05/2009 | H | Indiana State | W | 30 | 10 | 1-0 | 39,344 | Louisville | Ky. | Papa John's Cardinal Stadium | |||||
| 09/19/2009 | A | Kentucky | L | 27 | 31 | 1-1 | 70,988 | Lexington | Ky. | Commonwealth Stadium | |||||
| 09/26/2009 | A | RV | Utah | L | 14 | 30 | 1-2 | 45,588 | Salt Lake City | Utah | Rice-Eccles Stadium | ||||
| 10/02/2009 | H | Pittsburgh | L | 10 | 35 | 1-3 | 0-1 | 39,948 | Louisville | Ky. | Papa John's Cardinal Stadium | ||||
| 10/10/2009 | H | Southern Mississippi | W | 25 | 23 | 2-3 | 0-1 | 37,268 | Louisville | Ky. | Papa John's Cardinal Stadium | ||||
| 10/17/2009 | A | Connecticut | L | 25 | 38 | 2-4 | 0-2 | 40,000 | East Hartford | Conn. | Rentschler Field | ||||
| 10/24/2009 | A | 5 | Cincinnati | L | 10 | 41 | 2-5 | 0-3 | 35,099 | Cincinnati | Ohio | Nippert Stadium | |||
| 10/31/2009 | H | Arkansas State | W | 21 | 13 | 3-5 | 0-3 | 21,497 | Louisville | Ky. | Papa John's Cardinal Stadium | ||||
| 11/07/2009 | A | RV | West Virginia | L | 9 | 17 | 3-6 | 0-4 | 55,334 | Morgantown | W.Va. | Milan Puskar Stadium | |||
| 11/14/2009 | H | Syracuse | W | 10 | 9 | 4-6 | 1-4 | 33,223 | Louisville | Ky. | Papa John's Cardinal Stadium | ||||
| 11/21/2009 | A | South Florida | L | 22 | 34 | 4-7 | 1-5 | 49,388 | Tampa | Fla. | Raymond James Stadium | ||||
| 11/27/2009 | H | Rutgers | L | 14 | 34 | 4-8 | 1-6 | 23,422 | Louisville | Ky. | Papa John's Cardinal Stadium |